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Journal: The Journal of Biological Chemistry
Article Title: The Molecular and Functional Interaction between ICln and HSPC038 Proteins Modulates the Regulation of Cell Volume
doi: 10.1074/jbc.M111.260430
Figure Lengend Snippet: HSPC038 knockdown impairs ICl swell . HSPC038 knockdown was realized by using specific siRNAs in HEK293 Phoenix cells. a , the levels of the transcripts of HSPC038 and of the housekeeping gene β -actin were detected by RT-PCR in three independent samples of siRNA or control-siRNA (control)-treated cells. −RT , as a control, the PCR was conducted on a sample of total RNA that was not subjected to the reverse transcription reaction, to exclude genomic DNA contamination. b , densitometric analysis of the HSPC038 signal normalized to the β -actin signal. ***, p < 0.001 between siRNA-treated cells and control siRNA-treated cells, unpaired t test. ICl swell activation was monitored after hypotonic shock in siRNA-treated cells or in control cells. c and d , the experiments were done in the whole cell configuration; the current density-to-voltage (c) and the current density-to-time ( d ) relationships are shown. The data were fitted with second order polynomials following application of the extra sum of squares F test ( c and d : siRNA versus control: p < 0.0001).
Article Snippet: 1 μg of total RNA was used for the reverse transcription reaction with the QuantiTect® reverse transcription kit for cDNA synthesis with integrated removal of genomic
Techniques: Knockdown, Reverse Transcription Polymerase Chain Reaction, Control, Reverse Transcription, Activation Assay